http://rdf.ncbi.nlm.nih.gov/pubchem/patent/LU-500008-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e0a0a22e173e411bf7f25e395dacbdb |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-305 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6439 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
| filingDate | 2021-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3110885914e90eb30c8fd482dca12985 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cfe10b5a8d96b80975539927a3f29d66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd8f5a2db8013609d6c0c84988c80cd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3597506b21998fc76ac7c6f5f471498d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c74028763be75d5a6bfa2c504cdb3b1c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_987d943eb9624144c6e2fe3df8ab42aa |
| publicationDate | 2021-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | LU-500008-B1 |
| titleOfInvention | Animal tissue clearing method |
| abstract | The present invention provides an animal tissue clearing method, and belongs to the technical field of animal tissue observation. The method includes the following steps: (1) anesthetizing an animal, sequentially perfusing heart perfusate and a tissue fixation solution to a heart of the animal, then dissecting the animal, and separating animal tissue; (2) placing bone tissue in a decalcification solution, and performing decalcification for 5-12 d to obtain decalcified bone tissue, where solution change is conducted once every 1-3 days in the decalcification process; (3) respectively placing decalcified bone tissue and soft tissue in a destaining solution, and performing destaining to obtain destained tissue; (4) placing the destained tissue in a degreasing solution, performing gradient degreasing, placing degreased tissue in a dehydration solution, and performing dehydration to obtain dehydrated tissue; and (5) placing the dehydrated tissue in a clearing solution, and performing clearing treatment so as to obtain clear tissue. |
| priorityDate | 2020-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.