Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cf30310b38829a0aaa026014d975bc29 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-305 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-302 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2001-2873 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-286 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-312 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-34 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
filingDate |
2020-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2021-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2cffce4b3a997066ef23f729bf3d4573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ec2c17856e57450b7b9431bd7cd6499 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1457ecdc115065ab1cc95d27e9b0c44e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50075bf7c48ff2dfb8566aab806c41f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a446ee193c002659c50af6786c30d322 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_76c882f9d9ffbcf2ffd7a87ec6a761ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45d4c22047e5d7d8ea6a54a39202bd84 |
publicationDate |
2021-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
LU-101831-B1 |
titleOfInvention |
A Method for Staining Adipocytes and Application Thereof |
abstract |
The invention discloses a method for staining adipose tissue, comprising steps of a. using TritonX-100 to treat frozen sections of adipose tissue; b. removing TritonX-100; c. fixing the sections with glutaraldehyde; d. staining the sections with oil red O; e. decoloring the sections; f. using DMSO to treat the sections; g. removing MSO; h. staining the sections with Coomassie Brilliant Blue R250; i. decoloring.Such method is used for staining cytoskeletons. The invention discloses another method for staining adipose tissue, comprising steps of a. using DMSO to treat frozen sections of adipose tissue; b. removing DMSO; c. using TritonX-100 to treat the sections; d. removing TritonX-100; e. fixing the sections with glutaraldehyde; f. removing glutaraldehyde; g. staining the sections with Coomassie Brilliant Blue R250; h. decoloring. The invention also discloses two kits for staining test by such two methods and application thereof. |
priorityDate |
2019-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |