abstract |
The present invention provides a microassay card comprising an upper layer containing a groove for containing a liquid sample. The second layer of cards below the first layer includes a support surface bonded to the reactive species. The third layer comprises a superabsorbent support saturated with an indicator. Typically, the indicator is a substrate for the enzyme, such as a reduced dye precursor and a hydrogen peroxide generator, necessary for the action of the enzyme on the substrate to produce a spectral change in the absorption layer. The microassay card of the present invention, in which the card can be formatted for displacement or capacitive assays by selecting the structure of the first and second layers, is particularly useful for drug testing. |