http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-930023463-A
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-61 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K1-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-61 |
filingDate | 1992-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 1993-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | KR-930023463-A |
titleOfInvention | Method for Purifying Chicken Growth Hormone Expressed in Escherichia Coli |
abstract | In the present invention, in purifying chicken growth hormone expressed in genetically recombined Escherichia coli, (A) E. coli, which is expressed in chicken growth hormone, is isolated to separate insoluble chicken growth hormone refractors, and (B) separated insoluble refractors Washed with a solution containing a surfactant and ethylenediaminetepraacetic acid and then again with 100-500 mM sodium chloride or distilled water, and (C) the insoluble refractor was dissolved at pH11.5-13.0 with sodium hydroxide, (D) removing undissolved precipitates by centrifugation and one ultrafiltration, and (e) purifying chicken growth hormone by anion exchange resin column chromatography to mass-purify chicken growth hormone in high purity and high yield at low cost. To provide a way. |
priorityDate | 1992-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 123.