| abstract |
The present invention relates to a method for producing mRNA having a 3'-poly (A) tail of 20 nt to 500 nt in length without including a poly (A) tailing step, etc., and the mRNA construct produced by the production method of the present invention is stable and However, it is expected to contribute to the active use of transient transformation technology as it can express a target protein that is injected into a cell and exhibits its function intact for a long time. In addition, the transient transgenic CAR-NK prepared by the method of the present invention has excellent cancer cell killing ability, and since in vivo safety is ensured by expressing CAR without gene insertion into the genome of NK cells, a pharmaceutical composition for anticancer and targeting solid cancer It can be usefully used for cancer immunotherapy. |