| abstract |
The present invention relates to methods, cells and compositions for producing a product of interest, such as a recombinant protein. In particular, the present invention provides improved mammalian cells expressing a product of interest, wherein such cells (eg, Chinese Hamster Ovary (CHO) cells) express certain host cell proteins, such as certain lipases, esterases reduced or eliminated activity, eg, expression, of enzymes, including, but not limited to, hydrolases, and/or hydrolases. |