| abstract |
Provided herein are methods of monitoring the production of multispecific binding proteins and one or more mispaired species by a cell line, and methods of production and screening related thereto. In some embodiments, the method comprises an amount (e.g., relative amount) of a multispecific binding protein and one or more mispaired species in the cell culture medium by size exclusion ultra-high performance liquid chromatography and mass spectrometry (SE-UPLC-MS). ) to detect. In some embodiments, the multispecific binding protein is a multispecific antibody, antibody fragment, or Fc fusion protein. |