abstract |
The present invention provides a method for preparing a wound treatment composition comprising the steps of; i) classifying the whole blood sample into a plurality of samples comprising a platelet rich plasma (PRP) sample, a platelet-poor plasma (PPP) sample and a red blood cell sample, wherein the PRP sample has a hematocrit level of 1-10%; ii) processing a portion of the PPP and/or PRP sample by promoting cleavage of the PPP and/or autologous prothrombin present in the PRP to produce autologous thrombin, and iii) treating the PRP sample to produce a wound treatment composition. combining a portion of the PPP sample and a portion of the thrombin produced in step (ii), wherein step ii) is performed at less than 15°C. In a preferred embodiment, the PRP has a hematocrit level of 2 or 8%. The wound treatment compositions produced by the methods are also provided as compositions for use in the treatment of chronic and acute wounds. |