| abstract |
Described herein include isolation of CD8+ T cells from a blood sample obtained from a patient or donor, culturing the isolated CD8+ T cells in the presence of one or more cytokines, multimers containing the target peptide in complexes with MHC molecules, and contacting a cultured CD8+ T cell with at least one binder that binds to a T cell surface molecule, wherein the multimer is labeled with a first detectable agent and the binding agent is labeled with a second detectable agent; 2 A method for producing T cells comprising sorting of contacted CD8+ T cells for collection of sorted CD8+ T cells that are detected as positive for a detectable agent and expansion of the collected CD8+ T cells. |