Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e91d17fba854fff0cf1f57bc47a8d2b5 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21088 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4703 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-6424 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 |
filingDate |
2020-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa6dd95c6470209a8cc5df85405632bb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99e0837aa0ecd4bc44830c84eeb26eb5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8dbd33d863bd5b87abc4be17de76729c |
publicationDate |
2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
KR-20220031070-A |
titleOfInvention |
Increased Nucleic Acid-Guided Cell Editing Via LEXA-RAD51 Fusion Protein |
abstract |
The present disclosure provides compositions and methods for increasing the percentage of edited yeast cells in a cell population when using nucleic acid-guided editing, and automated multi-module instruments for performing these methods. Thus, in one embodiment, a promoter directing transcription of an editing cassette comprising a guide nucleic acid and a donor DNA sequence; yeast origin of replication; bacterial origin of replication; a promoter that directs transcription of the coding sequence for the nuclease; a promoter that directs transcription of the selectable marker; one or more LexA DNA binding sites; and a promoter driving transcription of the LexA-linker-Rad51 fusion protein. An editing vector for nucleic acid-guided nuclease editing in yeast is provided. |
priorityDate |
2019-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |