| abstract |
The present invention provides a method for generating genetically modified T cells, comprising the steps of a) providing a sample comprising T cells, b) preparing said sample by centrifugation, c) enriching the T cells, d ) activating the T cell with a modulator, e) genetically modifying the T cell by transduction with a lentiviral vector particle, f) removing the modulator, whereby the genetically modified T and generating a sample of cells, wherein the method is performed in less than 144 hours, less than 120 hours, less than 96 hours, less than 72 hours, less than 48 hours, or less than 24 hours. In one embodiment of the present invention, said enrichment of T cells is performed by magnetic cell isolation using magnetic particles coupled directly or indirectly to an antibody or antigen-binding fragment thereof specific for CD4 and/or CD8, Here, the magnetic particles may be removed from the cells after separation. |