| abstract |
a) providing a template oligonucleotide (I) complementary to the sequence of the product, said template having properties such that it is isolated from the product; b) providing a pool of oligonucleotides (II); c) contacting (I) and (II) under conditions permitting annealing; d) denaturing the annealed template and impurity oligonucleotide strands and changing conditions to isolate any impurities, including isolating impurities; and e) modifying the annealed template and product oligonucleotide strands and changing conditions to isolate the product, comprising isolating the product. Disclosed herein are novel methods for the production of oligonucleotides suitable for use in production. |