| abstract |
Disclosed herein are methods and compositions for purifying antibodies. Purification is carried out by covalently binding protein A domain (E, D, A, B, C) or domain Z, or a functional variant thereof, with the Drosophila Drosophila transcription factor Ultrabithorax (Ubx) material to improve the binding capacity of protein A chromatography. do. The composition comprises a fusion protein or fragment thereof containing the transcription factor Ubx of Drosophila yellow and an immunoglobulin binding protein. In some embodiments, the immunoglobulin binding protein is a protein A domain, a protein Z domain, or a fragment thereof. |