| abstract |
An improved, safer and more efficient process for developing genetically engineered cells is described. More specifically, introducing a donor DNA construct, a guide RNA, and an RNA guided nuclease into a host cell to be transfected; and introducing the three components into a host cell. A donor DNA construct designed to insert a CAR (chimeric antigen receptor) into a defined genomic region of a host cell is further described. Additionally, the disclosure herein provides host cells transfected with a CAR in the absence of a viral vector that may present safety concerns. The disclosure herein provides a more efficient and less expensive method to engineer T cells to express CAR constructs. |