| abstract |
For improved selection of an efficient targeted genomic manipulation agent 310 , the device 102 is a nucleic acid sample 306 and a targeted genome functionalized to the capture surface 126 within the sensing range 328 of the sensing surface 308a. first and second chip-based biosensors (104a, 104b) having one or more sensing surfaces (308a) configured to detect biomolecular binding interactions between the manipulating agent (310). The first chip-based biosensor 104a uses a nucleic acid sample 306 incubated with a blocking agent 318, and the second chip-based biosensor 104b uses a nucleic acid sample 306 without the blocking agent 318. ) is accepted. Measurement instrument 102 measures first and second sets of response signals 342a, 342b indicative of respective biomolecular binding interactions. The analysis module 116 determines a genome manipulation efficiency parameter of the targeted genomic manipulation agent 310 . System 100 and method 300 perform the functions of device 102 . |