| abstract |
In some aspects, the present disclosure relates to a mutant tRNA in which the first letter of an anticodon is substituted with lysidine or agmatidine, and a translation system comprising the mutated tRNA. In a specific aspect, the present disclosure provides a mutant tRNA capable of selectively translating the codons of the NNA. In another aspect, the present disclosure provides a translation system capable of translating two or three types of amino acids from one codon box. In another aspect, the present disclosure provides a novel method for synthesizing lysidine diphosphate, agmatidine diphosphate and derivatives thereof. |