| abstract |
An augmented method for rapidly and cost-effectively analyzing microbial sequences by qPCR is disclosed. These methods identify allelic modifications, SNPs and genetic modifications of specific genes, such as causative genes that confer resistance or sensitivity to antibiotics, chemotherapy, or other chemical compounds. By selecting appropriate genomic regions, mutations conferring resistance to major antibiotics can be identified by qPCR. In addition, the method can identify heterologous-resistant strains in a sample, eg, a population of strains comprising both mutant and wild-type nucleotides. By selecting the appropriate one that binds effectively to the mutant region, resistance conferring mutations can be identified. The method is useful for viral material such as influenza virus, bacterial material such as tuberculosis bacteria, and sequences derived from cancer cells. |