http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20210083430-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f140c24f626576edd1510c41b0adab42 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-522 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-0275 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-112 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 |
| filingDate | 2019-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf434230aa59ac50770928696bbe764b |
| publicationDate | 2021-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20210083430-A |
| titleOfInvention | The separation method of anti-inflammatory lipid A and immunostimulatory core O-antigen polysaccharides from lipopolysaccharide of Salmonella Typhimurium mutation strain |
| abstract | The present invention relates to a method for isolating and purifying anti-inflammatory fat A and core O-antigen that promotes immune cell activity from lipopolysaccharide of a Salmonella typhimurium mutant strain with weakened virulence. In the present invention, lipopolysaccharide is safely extracted from cultured cells of a mutant strain with weakened virulence that synthesizes the same lipopolysaccharide as that of Salmonella Typhimurium strain, and 0.25% trifluoroacetic acid solution Fat A and core O-antigen polysaccharides were hydrolyzed by incubation at 50 °C, and fat A and core O-antigen polysaccharides were separated with a Sep-Pak tC18 cartridge, and the gel filtration conditions were optimized to reduce the endotoxin effect. A size exclusion chromatography method for purifying single fractions of fat A and core O-antigen polysaccharide with high efficiency and high purity is provided. And a method for inhibiting the inflammatory response of mouse macrophages and promoting immune cell activity using the purified fat A and core O-antigen polysaccharide will be described. |
| priorityDate | 2019-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 164.