| abstract |
The present invention is an assay that can be used to predict whether a given ISV will undergo protein interference as described herein and/or will generate a (non-specific) signal in an assay (e.g., ADA immunoassay). (Such predictive assays can be used, for example, to test whether a given ISV may have a tendency to generate such protein interference and/or such signal, and/or may be used to detect such protein interference or occurrence of such signal. It can be used to select ISVs that are less prone or less prone to suffer and/or to test whether some change(s) to the ISV will reduce (in whole or in part) this interference or its tendency to generate such signals. As an assay or test that can be used as an assay or test that can be used and/or can be used to guide the alteration or improvement of an ISV to reduce its tendency to generate such protein interference or signals. Can be); -A method of altering and/or improving the ISV to eliminate or reduce such protein interference or its tendency to generate such signals; -Alterations that can be introduced into ISVs that eliminate or reduce such protein interference or its tendency to generate such signals; ISVs specifically selected (e.g., by using the assay(s) described herein) to have no tendency to generate such protein interference or such signals or to have a (more) lower/reduced tendency; And-an altered and/or improved ISV that does not have a tendency to generate such protein interference or such signals, or has a (less) lower/reduced tendency, and in some specific non-limiting embodiments. |