http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20210027427-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0018 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 |
| filingDate | 2019-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20210027427-A |
| titleOfInvention | Method for producing recombinant protein |
| abstract | In certain embodiments, the disclosure comprises culturing a host cell expressing the recombinant polypeptide of interest in a non-perfusion-based culture system, wherein the viable cell density is increased to at least 5 x 10 6 cells/mL. It provides a novel method of increasing the viable cell density of N-1 large scale bioreactor cell culture. In certain embodiments, the disclosure is a step of (1) culturing a host cell expressing the recombinant polypeptide of interest in step N-1 of a non-perfusion-based culture system, wherein the viable cell density is at least 5 x 10 6 Increasing to cells/mL; And (2) culturing the N fed-batch producing cells in a medium enriched with a high seed density of at least 1.5 x 10 6 cells/mL, wherein the N fed-batch producing cells are N- It provides a novel method of producing a recombinant polypeptide of interest on a large scale, comprising the step of being inoculated from step 1. |
| priorityDate | 2018-07-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 200.