| abstract |
In the present invention, a method for purifying a disintegrin-like and metalloproteinase (ADAMTS13) recombinant protein having a thrombospondin type 1 motif 13 from a sample is presented. The method includes enriching the ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions such that the ADAMTS13 protein can appear in the eluate or supernatant from the hydroxyapatite. These methods may further include tandem chromatography with a mixed modal cation exchange/hydrophobic interaction resin that binds to the ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and virus inactivation. In addition, the present invention provides a method for inactivating viral contaminants in a protein sample, wherein the protein is immobilized on a support. In addition, the present invention provides a composition of ADAMTS13 prepared according to these methods. |