| abstract |
The present invention is a step of inducing dissociation of mat-VWF and VWF-PP by destruction of non-covalently associated mat-VWF and VWF-PP, wherein the dissociation is (i) at least one chelating agent added Or (ii) incubating the composition comprising the steps of, induced by increasing the pH to a pH of at least 7, and collecting the mat-VWF to obtain a high purity propeptide depleted mature VWF (mat-VWF). It relates to a method of separating mature von Willebrand factor (mat-VWF) from von Willebrand factor pro-peptide (VWF-PP) by treatment. |