http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20200142870-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a9c8be5926503f1c3cb4dbcce92afd13 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01001 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-01001 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-96 |
| filingDate | 2019-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b80e35bb14146a464cca1712fffa006c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_245207e62b4bebc05f31831f7500750e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7876b889bd56482dcb689151dcb3e967 |
| publicationDate | 2020-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20200142870-A |
| titleOfInvention | Enzyme immobilization method using porous calcium carbonate microsphere |
| abstract | The present invention relates to an enzyme immobilization method using porous calcium carbonate microspheres, and more particularly, to an enzyme immobilization method using porous calcium carbonate microspheres capable of reducing protein denaturation and improving storage stability of enzymes. will be. To this end, the enzyme immobilization method using porous calcium carbonate microspheres includes the first step of preparing a calcium chloride solution by adding and stirring calcium chloride to a solvent in which distilled water and acetone are mixed; A second step of adding sodium carbonate to distilled water and stirring to prepare a sodium carbonate solution; A third step of mixing and stirring the calcium chloride solution and the sodium carbonate solution to form a calcium carbonate precipitate; A fourth step of separating the calcium carbonate precipitate using a filtration filter; A fifth step of drying the separated calcium carbonate precipitate to prepare porous calcium carbonate microspheres; A sixth step of adding and stirring porous calcium carbonate microspheres to a PB (Phosphate buffer) solution to which a predetermined enzyme is added; A seventh step of ultrasonicating the PB solution to which the porous calcium carbonate microspheres are added; An eighth step of stirring the PB solution at room temperature at a speed of 200 to 300 rpm; A ninth step of centrifuging the PB solution to remove enzymes not adsorbed to the porous calcium carbonate microspheres; A tenth step of adding a new PB solution, adding a crosslinker, and leaving it for a predetermined time; An 11th step of stirring the PB solution at room temperature at a speed of 200 to 300 rpm; A 12th step of centrifuging the PB solution to remove enzymes and crosslinkers that are not adsorbed on the porous calcium carbonate microspheres; And a thirteenth step of storing the porous calcium carbonate microspheres to which the enzyme is fixed therein. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20220124846-A |
| priorityDate | 2019-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.