| abstract |
The present invention provides a 5-aminolevulinic acid synthase (ALA synthase), wherein the C-terminal amino acid residue of the ALA synthase is deleted or is not alanine, and/or the amino acid sequence sequence of the ALA synthase In No. 403 corresponding to the amino acid sequence represented by number 1, the amino acid residue is deleted or is not alanine, and/or 1 to N arbitrary amino acid residues are added to the C-terminus of the ALA synthase, and ALA synthase activity is Have. The present invention further provides a method for preparing the enzyme, and includes the expression carrier of the enzyme, the host cell, the application of the enzyme in the production of ALA, and a method of improving the ALA synthase to provide its activity. |