| abstract |
An isolated nucleic acid containing a nucleotide sequence encoding the polypeptide of SEQ ID NO: 3. The polypeptide of SEQ ID NO: 3 specifically binds to stage-specific embryonic antigen 4 (SSEA4). Recombinant cells comprising the isolated nucleic acid described above, a viral vector containing the isolated nucleic acid, and an isolated polypeptide comprising the sequence of SEQ ID NO: 3 are also disclosed. Also provided is a chimeric antigen receptor (CAR) comprising a single chain Fv that has the sequence of SEQ ID NO: 3 and specifically binds to SSEA4. Moreover, T cells of a subject having a tumor expressing SSEA4 are transduced in vitro with a vector encoding CAR, the transduced T cells are expanded, and the expanded and transduced T cells are injected into the subject, thereby Thereby causing an anti-tumor T cell response, a method of treating a tumor is disclosed. |