| abstract |
It relates to a composition for gene editing comprising a carrier-free multiple CRISP/Cas 9 gene editing complex for editing multiple target DNA and use thereof. According to the gene editing complex according to an aspect, in the formation of a complex with the guide RNA and the intracellular delivery of the guide RNA, even without other cationic polymers or lipid carriers, superior delivery of the guide RNA in vivo or in vitro compared to conventional methods It has activity, and it is possible to edit more than one gene effectively, so that it is possible to edit multiple loci at the same time. In addition, since the gene editing complex according to one aspect can effectively inhibit the expression of PD-L1 and PD-L2, it can effectively kill cancer cells by inducing the production of interferon-γ by T cells, so it can be usefully used as an anticancer agent. There is an effect. |