| abstract |
The present specification provides a serum-free medium for cultivation, such as culturing, preparing and/or producing cells, such as immune cells, such as genetically engineered cells. It also provides liquid basal media and frozen supplements that can be used to produce serum-free media. Provided embodiments include methods of preparing a serum-free medium and methods of culturing cells, such as activating, transducing, culturing or amplifying cells in the presence of a serum-free medium. |