| abstract |
Structures encoding chimeric antigen receptor (CAR) and truncated human epidermal growth factor receptor (huEGFRt) are described. The encoding CARs are in the CD8α hinge region, the CD8α transmembrane region, the 4-1BB co-stimulatory domain, and the CD3ζ signaling domain (CD3ζsignaling domain). Includes tumor antigen specific monoclonal antibodies, such as fused glycpican-3-specific ((glypican-3) (GPC3)-specific), GPC2-specific or mesothelin-specific monoclonal antibodies do. Isolated host cells are also described, such as isolated T cells co-expressing published CARs and huEGFRt. T cells transduced with the disclosed CAR constructs can be used for cancer immunotherapy. |