Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_85a514fa16377d871b4828cfc8a0faf0 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M1-264 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D61-146 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-066 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M47-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 |
filingDate |
2018-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa694ecd4dcbd1484459a3e27faa96b0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f6b2acc0fcb3d7546a0c6aeb4fc5561 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ca81d7eeac6383950244348ee8a3605 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af2ca270574128644b36c33d2d63d43e |
publicationDate |
2020-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
KR-20200034746-A |
titleOfInvention |
Semi-dry bead beating method for crushing microorganisms, and apparatus for performing the same |
abstract |
Methods and apparatus for crushing cells to release or extract genomic DNA (gDNA) are disclosed. The method utilizes a mixture of fine glass beads and cells (e.g., spores) that form a semi-dry cake that sticks to the sides of the tube and larger metal balls during bead beating crushing, greatly improving the efficiency of the bead beating process. . The device creates a disordered movement that ensures that sufficient force is generated to open the cells and that the metal ball impact is distributed across the inner surface of the container so that all cell mixtures are subjected to sufficient impact to cell destruction. As a result, spores and other hard-to-crush microorganisms can be opened in seconds. This method reduces the number of steps and the working time by quickly opening cells that are difficult to break while maintaining the integrity of the DNA. |
priorityDate |
2017-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |