| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c8c293fd340e0c8b6a445f7633453a0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0644
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-164
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-149
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0663
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0478
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-1805
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-087
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0864
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0861
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0816
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-0652 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 |
| filingDate |
2017-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58a7ddb684966eac1afdb89de47881e6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69ecc7015c4df33df506ffb51689273c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_824eda6010da93bbfc18b801785dde0b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c5683baf7c454587d1d1ed8359dc0c6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2edb5559e67b394cff5394a0a1332de6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eca5ce1a15aaba1da0e5b022e326445b |
| publicationDate |
2019-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-20190105224-A |
| titleOfInvention |
Integrated Purification and Measurement of DNA Methylation in Automated Reaction Cartridges and Simultaneous Measurement of Mutations and / or mRNA Expression |
| abstract |
Methods of determining methylation of DNA are provided. In one exemplary, but non-limiting embodiment, the method comprises the steps of i) contacting a biological sample comprising a nucleic acid with a first matrix material comprising a first column or filter, wherein the matrix material is added to the nucleic acid in the sample. Binding and / or filtration to purify DNA; ii) eluting bound DNA from the first matrix material and denaturing the DNA to produce eluted denatured DNA; iii) heating the eluted DNA in the presence of bisulfite ions to produce a deaminated nucleic acid; iv) contacting the deaminated nucleic acid with the second matrix material by contacting the deaminated nucleic acid with a second matrix material comprising a second column; v) desulfonating and / or simultaneously eluting bound deaminated nucleic acid and contacting the deaminated nucleic acid with an alkaline solution to desulfonate the nucleic acid to produce a bisulfite converted nucleic acid; vi) eluting said bisulfite converted nucleic acid from said second matrix material; And vii) performing methylation specific PCR and / or nucleic acid sequencing, and / or high resolution melt analysis (HRM) on the bisulfite-converted nucleic acid to determine methylation of the nucleic acid, at least step iv ) To vi) are performed in a single reaction cartridge. |
| priorityDate |
2016-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |