| abstract |
The present invention relates to a primer set comprising a primer represented by the following formula 1: [Formula 1] 5'-XYZ-3 ' Wherein X is a first binding site comprising a hybridization nucleotide sequence of 15 to 25 bp complementary to a template nucleic acid to be hybridized, Y is a ring forming site of 3 to 10 bp, and Z is a nucleotide sequence complementary to the template nucleic acid, 10 bp, and a second binding site comprising a nucleotide corresponding to an allele and a non-complementary nucleotide of 1 to 3 bp consecutive thereto, wherein X, Y and Z are deoxyribonucleotides or ribonucleotides. |