http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190053960-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_263975a1eaf1605697e372e77e0dc541 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2523-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0633 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2017-10-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3287e042088b97ef804510b7a0a20e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fcd205ab59f040546b0c76e3d2f4cae4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3aa1a435197038c767c880e0c1f993d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f857a68713cd6a61badaf15556f9f8b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fa62c3a8749b989ba0a05d35094a474 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ea1be6c3ff0fcaf6ab769d2524d3790 |
| publicationDate | 2019-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20190053960-A |
| titleOfInvention | Culture method of human salivary gland cells |
| abstract | The object of the present invention is to increase the number of submerged cultures of human salivary gland cells and to maintain undifferentiated state and high proliferation possibility during culturing. (a) obtaining human salivary epithelial progenitor cells from the recipient organism; (b) Cells were transferred to PCT epidermal keratinocyte culture medium, cultured in culture flask, 5% CO 2 was added, and the medium was changed every 2 to 4 days until a monolayer was formed, Assuring adhesion; (c) subculturing the cells at a dilution ratio of 1: 3 to 1: 5, except that the EDTA trypsin solution is used to separate the cells from the culture flask surface and then transferred to a new culture flask; ; (d) further performing cell culture as defined in step (b), wherein the intermediate medium is exchanged every 2 to 4 days, and a single layer as defined in step (c) Culturing human salivary epithelial progenitor cells containing submerged culture at a maximum dilution ratio of 1: 2 to 1: 3 until formation, wherein the culture medium first exchanged after each subculture should be performed within 8 to 24 hours] Method is provided. |
| priorityDate | 2016-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 400.