| abstract |
The present invention provides a method for growing an adhesion-dependent cell (e.g., hUTC) in a culture medium comprising an amino acid, a vitamin, a salt, a nucleoside, an insulin, a transferrin, an ethanolamine and sodium selenium, Is supplemented. The method further comprises the addition of serum-free nutrients including amino acids, vitamins, salts, nucleosides, insulin, transferrin, ethanolamine and sodium selenium. The present invention also provides a culture medium for adhesion-dependent cell growth and a serum-free nutrient solution. |