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publicationDate 2018-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20180106726-A
titleOfInvention Single stranded dna aptamers specifically binding to staphylococcal enterotoxin b
abstract DNA library containing DNAs having 40 to 60 bases randomly arranged in the center with primer regions for PCR at both ends, and Staphylococcus aureus B, a DNA library containing DNAs having 40 to 60 nucleotides randomly arranged in the center, were mixed in a binding buffer solution and reacted to form a Staphylococcus aureus toxin B- Introducing a graphene oxide-DNA conjugate by reacting graphene oxide with a mixed solution of the DNA library and Staphylococcus aureus toxin B, and introducing the DNA library, Staphylococcus toxin B and oxidized graphene Extracting the Staphylococcus aureus toxin B-DNA complex by centrifuging the mixed solution, separating the DNA from the Staphylococcus aureus toxin B-DNA complex, amplifying the separated DNA through a polymerase chain reaction, Mixing said amplified DNA and a plurality of non-target proteins into a binding buffer solution to form said plurality of non-target toxin-D (S6) of introducing an amplified DNA and a non-target protein into a mixed solution of the amplified DNA and a plurality of non-target proteins, and introducing the graphene oxide- - centrifuging a mixed solution of the target protein and the oxidized graphene to extract DNA that is not bound to the non-target protein, and discloses a method for producing an abatumer that specifically binds to Staphylococcus aureus toxin B .
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