http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20180105772-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3164a963ca21cec1151213cb1bb95e77 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-12007 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y113-12007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0069 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 |
| filingDate | 2017-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5a1fb6977faa01f4ee8d2a564b1c0d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a36ac87bb167c6ad83fcf4dfdd7b1ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18476024ce23f2a8c8caacbfc51c4e1c |
| publicationDate | 2018-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20180105772-A |
| titleOfInvention | Mutant Gaussia luciferase with enhanced bioluminescence intensity |
| abstract | The present invention relates to Gaussia luciferase variants modified to amplify the bioluminescence intensity of luciferase derived from Gaussia Princeps using site-specific mutations. More specifically, the amino acid sequence of Gaussia princeps Luciferase is prepared by substituting one or more amino acids of the 60th, 88th, 89th, 90th and 103th amino acids in the amino acid sequence of G Luc ( Gaussia princeps Luciferase) A polynucleotide encoding the mutant sequence, a recombinant vector comprising the polynucleotide, a transformant containing the recombinant vector, a mutant gene having improved bioluminescence intensity from the transformant, A method for producing cialucleasease, and a kit comprising the mutant gauri luciferase. G Luc ( Gaussia luciferase) is the most widely used secretory luciferase for cell-based reporter assays. It is the smallest among the various luciferase-derived luciferases, It has the advantage of being able to be used for effective monitoring by minimizing the influence of the structure. However, the wild-type G Luc has a disadvantage in that the emission signal size is not sufficient. Accordingly, the present invention can produce a mutant GUS luciferase having improved bioluminescence signal by mutating an amino acid residue that plays an important role in the amplification of bioluminescence signal of Gaussia luciferase, and can be used for cell monitoring and drug screening And the like. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023123509-A1 |
| priorityDate | 2017-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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