| abstract |
The present invention is directed to a mediator-antibody covalent attachment method that can be activated only after binding with a binding site on the mediator-avaible antibody. To this end, there is provided herein a mediator that is engineered to contain a photoreactive functional group at one or more specific positions (s) on the mediator so as not to interfere with the specific non-covalent molecular interactions with the binding site on the mediator- But still allow and improve covalent bond formation. This methodology, with its speed, accuracy, consistency and simplicity, has obvious advantages for the development of antibody-drug conjugates. |