| abstract |
The present invention relates to an IL2RG gene knockout immunodeficient animal model and a method for producing the same, and more particularly, to an immunodeficient animal model using a recombinant vector for preparing immunodeficient animal models including sgRNA (small guide RNA) and Cas9 gene, And a manufacturing method thereof. The recombinant vector using the specific sgRNA and the CRISPR / Cas9 method for knocking out the IL2RG gene according to the present invention can effectively induce immunodeficiency by knocking out the DNA strand encoding the IL2RG gene in the transformed animal. Therefore, the IL2RG gene knockout animal model according to the present invention can be used for a variety of purposes such as identification of mechanisms of related diseases, development of therapeutic substances, development of diagnostic methods, development of regenerative medicine, development of a method for inhibiting heterologous transplantation adverse reactions, , An agent for inhibiting organ transplantation, an agent for treating cancer, and an agent for improving or treating SCID (Severe combined immunodeficiency). |