| abstract |
The present invention relates to a cell-free DNA detecting and recovering magnetic nanostructure comprising a conductive polymer and a cationic polymer on which magnetic nanoparticles are mounted. The cell-free DNA detecting and recovering magnetic nanostructure including the conductive polymer and the cationic polymer on which the magnetic nanoparticles according to the present invention are mounted can be used for a small amount of urine, cerebrospinal fluid, plasma, blood, Various types of cell free DNA (cfDNA) can be efficiently detected from biological samples. More specifically, since a strong magnetic field generated through mounting of a large amount of magnetic nanoparticles can efficiently capture the cell glass DNA present in a trace amount in the sample, and in the detection, separation and recovery of cell glass DNA, Due to the long structure of the wire structure, it is possible to attach a large amount of cationic polymer, polyethyleneimine, so that the efficiency of capturing the DNA present in the sample can be greatly enhanced. As a result, the detection sensitivity is improved, And the detection and recovery of the cell free DNA. In addition, the thin, elongated structure of the magnetic nanowire has a significantly improved detection efficiency because it can penetrate a large amount of cells or proteins present in the blood or urine and can effectively detect and capture cancer-related cfDNA. Therefore, the cell-free DNA detecting and recovering magnetic nanostructure according to the present invention is expected to be used for early diagnosis and treatment of cancer as well as for DNA mutation diagnosis by extracting DNA from blood cancer cells. |