abstract |
The present invention relates to a cloning vector comprising a novel non-mammalian virus-derived 2A sequence, and a polynucleotide for carotenoid biosynthesis and a transgenic plant. More specifically, the invention relates to a cloning vector comprising a non-mammalian virus derived 2A sequence, a phytoene synthase, a non-mammalian virus derived 2A sequence, a carotene unsaturated enzyme, a beta carotene hydrolase, and a poly Nucleotide, a recombinant expression vector comprising the polynucleotide, a transformant cell into which the polynucleotide is introduced, and a transgenic plant into which the polynucleotide has been introduced. The 2A sequence according to the present invention shows higher ribosome skipping efficiency than the conventional FMDV-derived 2A sequence, thus enabling the development of transgenic plants having high gene expression stability. In addition, the ribosome scumming 2A sequence according to the present invention is expected to increase public acceptance in the development of transgenic plants derived from non-mammalian viruses. |