| abstract |
A genetically modified mouse is provided in which the mouse is unable to rearrange and express the endogenous mouse immunoglobulin light chain variable sequence and the mouse is a human immunoglobulin sequence operably linked to a mouse kappa (kappa) constant gene in an endogenous mouse K locus Wherein the mouse expresses only one or two human light chain variable domains expressed by the human heavy chain variable region domain and the mouse light chain variable domain and mouse κ constant domain derived from only one of the two human light chain variable region gene segments, Expressing an inverted chimeric antibody having a variable domain and a mouse heavy chain constant domain. Binding epitope-binding protein that is completely human, and comprises two other heavy chains associated with the same light chain comprising a variable domain derived from one of two different human light chain variable region gene segments. |