| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2d4ad61d0af1fd483d31d1c5df9b9b76 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-45
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-155
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-998
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-33
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-415 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0621
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0018 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 |
| filingDate |
2016-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_877dab465edde17821831cc1197976ca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c18c857d75f93ea7b2c4c2965abda8c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6def0d5ff9f5157f80df8d4fdfd88762
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93adca72f9ebd061a30d1a85bcf585a3 |
| publicationDate |
2018-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
KR-20180017704-A |
| titleOfInvention |
Method for Culturing Cornea Stem Cell Like Cell by Inducing Differentiation of Induced Pluripotent Stem Cell Using Protein Ligand and System for the Same |
| abstract |
The present invention relates to a method for culturing a corneal stem cell-like cell line by inducing differentiation of an Induced Pluripotent Stem Cell, which method comprises culturing the corneal stem cell-like cell line with DMEM F12, L-ascorbic acid, sodium selenite, Preparing a basic medium containing the medium; Adding an inducer for culturing an inducible pluripotent stem cell to the basal medium; Preparing a feeder-free culture medium by coating the basic medium with the vitronectin recombinant human protein; Adding the inducer pluripotent stem cells to the feeder-containing medium; Adding an additive for stem cell growth environment composition to the feeder-containing medium; A step of sequentially adding BMP4 and Wnt3a to the feeder-containing medium to induce the induction of pluripotent stem cells into corneal stem cell-like cell lines; And culturing the cells differentiated from the induced pluripotent stem cells in a PI culture medium. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20210110127-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3871697-A1 |
| priorityDate |
2016-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |