| abstract |
The present invention relates to a group of glycosyltransferases and their applications. Specifically, the glycosyltransferases include enzymes gGT25, gGT13, gGT30, gGT25-1, gGT25-3, gGT25-5, gGT29, gGT29-3, gGT29- 4, gGT29-5, gGT29-6, gGT29-7, 3GT1, 3GT2, 3GT3, 3GT4 and derivatives thereof provide a catalytic function for the glycosylation of terpenoid compounds and applications in the synthesis of new saponins, Here, the glycosyltransferase enzyme can specifically and effectively catalyze the hydroxyl group glycosylation of the C-20 and / or C-6 region and / or C-3 of the tetracyclic triterpenoid based compound substrate, And / or the glycosyl group provided in the glycosyl group donor is transferred onto the first glyco group at the C-3 region of the tetracyclic triterpenoid compound to extend the oligosaccharide. The glycosyltransferase enzyme of the present invention may be applied to the artificial synthetic rare ginseng saponin and various new ginseng saponins and derivatives thereof. |