http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20170125801-A

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publicationDate 2017-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber KR-20170125801-A
titleOfInvention Mitochondrial collection and concentration, and uses thereof
abstract Methods and preparations for preparing concentrated mitochondrial preparations using reverse phase centrifugation are provided. The method includes centrifuging a closed bottom tube containing a heavy phase in the proximal tube section relative to the rotational axis and a light phase in the distal tube section relative to the rotational axis. Centrifugation causes the suspension to form a thickened mitochondrial layer at the interface between the heavy and hard layers, and collects the fluid at this interface to produce a concentrated mitochondrial formulation. Concentrated mitochondrial preparations can be used in a variety of applications, including in vitro fertilization.
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