| abstract |
The present invention relates to a negative single-stranded RNA comprising the following (1) to (8), a single-stranded RNA binding protein, a stealth-type RNA gene consisting of a RNA- By using an expression system, it is possible to simultaneously and stably express a plurality of foreign genes. (1) a target RNA sequence encoding an arbitrary protein or functional RNA, (2) an RNA sequence derived from an mRNA expressing in an animal cell constituting a noncoding region, (3) an RNA sequence derived from an RNA- (4) a transcription termination signal sequence recognized by the enzyme, (5) an RNA sequence comprising a replication origin recognized by the enzyme, (6) an RNA sequence encoding the enzyme, (7) an RNA sequence encoding a protein that regulates the activity of the enzyme, an RNA sequence in which the codon is optimized for the species from which the target cell is derived, (8) - An RNA sequence encoding a strand RNA binding protein whose codon is optimized for the species from which the target cell is derived. |