| abstract |
Disclosed is a method for producing a recombinant human α-galactosidase A (rh α-Gal A) at a high purity on a large scale. The method comprises the steps of (a) culturing rh α-Gal A-producing mammalian cells in serum-free medium, (b) collecting the culture supernatant, (c) applying a culture supernatant to anion-exchange column chromatography Column chromatography using hydrophobic column chromatography, (e) affinity for phosphate groups as a solid phase, (f) cation-exchange column chromatography, (g) A chemical column chromatography step, and (h) a gel filtration column chromatography step. |