| abstract |
The present invention relates to a method for producing a plant, comprising the steps of: obtaining, as a transient expression target, a complete plant that transiently expresses a nuclease different from a target gene fragment in a plant; Under the action of a nuclease, cutting the target fragment; And performing a site-specific transformation of the target fragment through DNA repair of the plant. The present invention also provides a method for performing a site-specific transformation on a target gene fragment in a whole plant through transient expression of the gene. The present invention also provides a method for producing a non-transgenic mutant plant by the above-mentioned method, whereby a non-transgenic mutant plant is provided. |