abstract |
The present invention relates to a method and a kit for detection of a cochlear polyclicoids using nuclease protection assay integrated sandwich hybridization, and more particularly to a nuclease protection assay probe, a capture probe, A method for detecting Cochlonidium polyclicoids contained in a sample by performing a sandwich hybridization integrated with a nuclease protection assay (NPA-SH) using a signal probe and a signal probe Kit. According to the present invention, it is possible to detect Cochrlidium polyclicoids from the sample very accurately in different microalgae, and quantitative detection is also possible. Therefore, it is expected to predict the occurrence of Cyclodinium polychloroides, and it will be a great help to maintain a stable marine ecosystem, and it will contribute to reduce unpredictable damage such as fisheries and aquaculture. |