| abstract |
The present invention relates to a method for detecting and quantifying a specific protein existing in a mixture sample by using a fluorescence polarization immunoassay on the basis of a droplet-based microfluidic system. Compared to a conventional scheme, the method according to the present invention can reduce a sample demand amount by minimum 1,000 times or more. Since separate sample fixing, washing, and separating steps are not required, deformation of a protein can be prevented and analysis can be performed in a short time. Moreover, even when a sample demand amount is reduced by minimum 1,000 times or more, a protein can be detected and quantitatively analyzed at the same level as conventional technology. Accordingly, a mixture sample of blood, urine, tears, saliva, body fluid, and foods can be more economically analyzed, and thus the method of the present invention can be useful in diagnosing diseases and analyzing food quality. |