| abstract |
The present invention provides a method for preparing a fructose solution comprising: preparing a fructose solution for an enzyme reaction; Performing an enzymatic reaction on the fructose solution and then heat-exchanging and cooling the D-cycozu solution to purify the ion; Concentrating the purified D-cycozu solution from which the impurities have been removed, subjecting the D-Cycoç solution to heat exchange cooling to separate it by continuous chromatography; Subjecting the continuous chromatographic separation mother liquor to heat exchange cooling to recycle it in the process; Concentrating the D-cicosol solution separated by the continuous chromatography to effect heat exchange cooling to crystallize; Dissolving the mother liquor and heat-exchanging and cooling the crystallization-separated mother liquor, thereby producing a high-purity D-psicose having a purity of 99% (w / w) or more. According to the process for producing D-psicose of the present invention, heat-denaturation of D-psicose is prevented by controlling the temperature in the process using a heat exchanger, and D-psicos can be produced with high yield by a stabilized process. In addition, the continuous chromatographic separation of fructose mother liquor and crystallization separation mother liquor is recovered and added to the enzyme reaction process, so that stable separation of D-cyclos can be achieved by continuous chromatography even after long-term recycling, Can be realized. |