http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20160053885-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cbffa3b81b3b862dc7220b5648f5f745 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4702 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-01164 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 2016-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35e5e392df8904b4542f241c4a5009e5 |
| publicationDate | 2016-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | KR-20160053885-A |
| titleOfInvention | Method for Producing Phosphoserine Incorporated Proteins by Using SepRS Mutants and EF-Tu Mutants |
| abstract | The present invention relates to a method for producing a phosphorylated protein using an activity-increasing SepRS ( O- phosphoseryl-tRNA synthetase) mutant and an EF-Tu mutant. More particularly, the present invention relates to a method for producing at least one Force in the tRNA Sep screened through a Sep tRNA and molecular evolution techniques to recognize a codon porcelain (phosphoserine, Sep) the amino quinoa misfire sikineunde specialized O - phosphonate Sheryl -tRNA synthetase (O-tRNA synthetase -phosphoseryl, SepRS) mutant And a method for preparing a phosphorylated protein by incorporating phosphocelene at a specific site using an EF-Tu mutant that protects the tRNA Sep and SepRS complexes from deacylation. According to the present invention, it is possible to produce phosphorylated proteins with mg yield per L using SepRS and EF-Tu mutants, and it is possible to produce various phosphorylated proteins including phosphorylase, antibody production, protein chip preparation, Screening and the like. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10723760-B2 |
| priorityDate | 2016-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 640.